RNA
Part:BBa_M36134:Design
Designed by: Daniel McHugh Group: Stanford BIOE44 - S11 (2012-06-01)
Design Notes
The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. The PP7 aptamer was omitted to decrease the length of sequence not involved in scaffold folding. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts.
Sources
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).
M. N. Win, C. D. Smolke, “A modular and extensible RNA-based gene-regulatory platform for engineering cellular function” PNAS 36, 14283-14288 (2007).